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Short stay

Aparecida Maria

Genetics - Brazil

Research topics


Development of a mRNA-based vaccine against SARS-COV-2 encoding multiple epitopes and using Lipo Particle as innovative carrier

Recently, twomRNA-based vaccines have been crucial for ending the ongoingseverepandemic of COVID-19. However, these vaccines encode only the spikeglycoprotein of SARS-CoV-2 and as the spikegeneis a mutation hotspot, hence at risk of losingsome of theirefficacywhen SARS-CoV-2 variantsemerge. Additionally, thesecurrentmRNA vaccines utilizelipidnanoparticletechnology,howeverhybrid carrier systemsprovide more flexibility and greaterfunctionalitycompared to non-hybridsystems. Withthis in mind, the proposedproject has 3 main objectives: 1) formulating a mRNA-based vaccine thatprovides protection against the SARS-CoV-2 pathogeninduced in response to antigensfromthree structural proteins; 2) includesignificant modifications of the mRNA structure in the production of IVT-mRNA to improvemRNA translation efficiency and overcome the innateimmunogenicity and 3) use LipoParticle as delivery system whichallowthe use of low doses of mRNA and enhanceendosomal escape.

Activities / Resume


Aparecida Maria Fontes is Assistant Professor at Genetics Department of Ribeirão Preto School of Medicine of University of São Paulo. Dr. Fontes interest is genetherapy, epigenetics and mRNA vaccine.